Restriction endonuclease analysis of Aspergillus fumigatus DNA.
نویسندگان
چکیده
منابع مشابه
Restriction endonuclease analysis of Aspergillus fumigatus DNA.
AIMS To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA ...
متن کاملRestriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans.
Mitochondrial DNA of the ascomycete fungus Aspergillus nidulans, a circular molecule of 31 500 base pairs, is cleaved by restriction endonucleases Eco R I, Hind II, Hind III and Bgl II into 3, 7, 9 and 5 fragments, respectively. The relative positions of the cleavage sites could be mapped by analysis of fragments obtained by double enzyme digestions of whole DNA and by complete and partial redi...
متن کاملGlobal Population Genetic Analysis of Aspergillus fumigatus
Aspergillus fumigatus is a ubiquitous opportunistic fungal pathogen capable of causing invasive aspergillosis, a globally distributed disease with a mortality rate of up to 90% in high-risk populations. Effective control and prevention of this disease require a thorough understanding of its epidemiology. However, despite significant efforts, the global molecular epidemiology of A. fumigatus rem...
متن کاملAnalysis of promoter function in Aspergillus fumigatus.
The filamentous fungus Aspergillus fumigatus is an important opportunistic pathogen that can cause high mortality levels in susceptible patient populations. The increasing dependence on antifungal drugs to control A. fumigatus has led to the inevitable acquisition of drug-resistant forms of this pathogen. In other fungal pathogens, drug resistance is often associated with an increase in transcr...
متن کاملRestriction Endonuclease Analysis of Pasteurella Multocida
In this study the restriction endonuclease analysis (REA) technique was used for the comparison of field isolates and vaccinal strain of Pasteurella multocida using Hha-I enzyme. On the basis of DNA finger prints, extent of homology among the field isolates and the vaccinal strain was observed. The study has provided the clear evidence that eight isolates of P. multocida used were serologically...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1992
ISSN: 0021-9746
DOI: 10.1136/jcp.45.4.324